The standard defines a list of 18 chemicals. The minimum breakthrough time for a Type A glove is 30 mins (Level 2) for 6 chemicals, for a Type B it is 30 mins for at least 3 chemicals, and for Type C it is 10 mins (Level 1) for at least 1 chemical on the list.
The chemical resistant glove pictogram must be accompanied by code letters for the tested chemicals for Type A and Type B gloves. Type C marked gloves are without any code letter.
List of chemicals:
Letter code Chemical CAS number Class A Methanol 67-56-1 Primary alcohol B Acetone 6764-1 Ketone C Acetonitrile 75-05-8 Nitrile compound D Dichloromethane 75-09-2 Chlorinated hydrocarbon E Carbon disulphide 75-15-0 Organic compound containing sulphur F Toluene 108-88-3 Aromatic hydrocarbon G Diethylamine 109-89-7 Amine H Tetrahydrofurane 109-99-9 Heterocyclic ether I Ethyl acetate 141-78-6 Ester J n-Heptane 142-85-2 Saturated hydrocarbon K Caustic soda 40% 1310-73-2 Inorganic base L Sulphuric acid 96% 7664-93-9 Inorganic mineral acid
M 65% Nitric acid 7697-37-2 Inorganic mineral acid, oxidizing N 99% Acetic acid 64-19-7 Organic acid
O 25% Ammonium hydroxide 1336-21-6 Organic base
P 30% Hydrogen peroxide 7722-84-1 Peroxide
S 40% Hydrofluoric acid 7664-39-3 Inorganic mineral acid, contact poison T 37% Formaldehyde 50-00-0 Aldehyde
EN 374-5: 2016 Protection against micro-organisms
EN 374-4: 2013 Resistance to chemical degradation
EN 16523-1: 2015 (replaces EN 374-3) Resistance to chemical permeation
Micro-organisms are defined by the standard as bacteria, fungi or viruses. To claim resistance to bacteria and fungi the glove must pass the penetration resistance test according to standard EN 374-2: 2014. If the glove passes ISO 16604: 2004 (method B) test it can claim resistance to viruses as well, and the term VIRUS will be added below the biohazard pictogram.
Degradation is the deleterious change in one or more properties of a protective glove material due to contact with a chemical. Indications of degradation can be delaminating, discoloration, hardening, softening, dimensional change, loss of tensile strength, etc. It is determined by measuring the percentage change in puncture resistance of the glove material after a continuous contact for 1 hour of the external surface with the challenge test chemical. The results of the degradation test must appear in the information leaflet for all three glove types.
Test method to measure the resistance of the PPE material to permeation by hazardous chemicals at molecular level and under continuous contact. The resulting value is the breakthrough time or the time needed by the hazardous liquid or gas to get in contact with the skin. The glove is classified in terms of breakthrough time performance level 1 to 6.
Measured breakthrough time
Permeation performance index
> 10 1 > 30 2 > 60 3 > 120 4 > 240 5 > 480 6
Type of gloves Breakthrough time
A ≥30 min for at least 6 chemicals
B ≥30 min for at least 3 chemicals
C ≥10 min for at least 1 chemical
EN ISO 374-5 EN ISO 374-5
A B C
IA L S